Where's the photomicrography thread?
Posted 11 February 2014 - 02:00 AM
Heres a video I took today. Ciliate twist.
Posted 03 March 2014 - 09:15 PM
Posted 08 April 2014 - 03:27 PM
Posted 12 April 2014 - 10:14 PM
Posted 13 April 2014 - 12:34 PM
Posted 18 April 2014 - 02:25 AM
Testing was done on a commercially prepared blood smear slide. Views of other slides looked fine, but they didn't have subject material that pops in phase.
Follow-up: I called Amscope, and talked to a specialist who said they can ship me a pre-tested PCT. I just emailed my purchase information to Amscope.
Posted 26 April 2014 - 06:03 PM
Here are some Springtails. I put some damp soil in a funnel with a small glass bottle filled with rubbing alcohol underneath. Then I drove the microscopic Springtails out of the soil with a light bulb placed above the funnel. I'll get better photos when I get an eyedropper or pipette and solidly mount them. This is 40X through the Olympus stereo scope.
Posted 28 April 2014 - 12:03 AM
Posted 28 April 2014 - 12:37 AM
I took the Springtail photos through my Olympus SZ4540 stereo microscope. I haven't critically examined photos taken through the T490, as I'm still experimenting.
I considered buying the $90-$100 Amscope DSLR microscope adapter to get parfocal performance in the T490, but the adapter gets poor reviews because of severe chromatic aberration. Above all, I want the best image quality, so I'm using a $15 no-optics Celestron 1.25" telescope adapter shimmed with plastic from a plastic cup. I slide it over the T490's trinocular tube sans eyepiece. I have to focus either for eyepiece or camera. Results should be better than with the B100, because the camera focal plane should be squared more easily with the T490 setup.
Posted 28 April 2014 - 02:15 PM
More on my PCT. I put 10X achro and Plan achro phase objectives side-by-side in the objective turret, and aligned all four condenser turret annular rings with the phase objective. I compared brightfield and phase views at each condenser turret position. Then I repeated the testing with 40X achro and Plan achro phase objectives
Conclusion: The condenser turret was faulty. Positions 1 and 2 could not give phase contrast views. Phase positions 3 and 4 gave worse views than brightfield, regardless of the phase objective used.
Posted 06 May 2014 - 05:54 PM
Posted 08 May 2014 - 12:01 AM
They didn't ship my replacement PCT today. They've had 6-1/2 working days to check the defective kit they received Apr. 28, and test and send me a working replacement. Tomorrow will be an ugly day for some Amscope employees.
*They shipped the replacement PCT yesterday, but the shipping dept. didn't follow instructions to notify me. ETA is tomorrow.
Posted 09 May 2014 - 07:52 PM
Are you in the east or north bay? Maybe I could use your microscope to compare with, and test the PCT with your scope?
Posted 11 May 2014 - 12:27 AM
Here are some microscopic insects @200X. Springtails (1 and 2), brown mite, dot bug underside.
Posted 12 May 2014 - 07:58 PM
The 200X photos show the PC view is semi-darkfield, and also without useful PC effect.
The 400X photos are where the benefits of PC should be most apparent, but I can't see any improvement in details in the right side (PC) photos.
There are some focusing issues in the photos, but no fine focusing adjustments made the PC views better than the BF views. I did some blind testing with my stepdaughter (an Industrial Biotechnology major) and she unfailingly called the PC views worse than the corresponding BF views.
Posted 29 June 2014 - 10:20 AM
My Amscope rep went well beyond excellent customer service, and got her manager to give me a courtesy refund for the lost PCT (I kept the T490A). I can't recommend Amscope's PCT, but the company has great customer service!
Posted 01 July 2014 - 04:08 AM
Posted 08 July 2014 - 03:01 PM
Posted 08 July 2014 - 03:05 PM
Posted 09 September 2014 - 12:14 PM
Here's a comparison between normal bright field, and bright phase contrast (as opposed to dark phase contrast - yes, I have both), using a living diatom at 200X for a subject. First, bright field:
And phase contrast:
Polarized light is a ton of fun, here's some Galveston beach sand with a couple forams (microscopic sea shells) in POL at 100X:
Observing sand is a lot like observing the sky; just as we occasionally run across unique stars among the myriad others, there are unique sand grains, like this cross-striated one seen at 400X:
How about a little sugar with that? Here's some table sugar that was dissolved in tap water, then a drop allowed to dry on a slide, then observed under POL at 100X:
Similar treatment applied to a Vitamin C tablet produced this crystal structure seen under POL at 100X:
Photos above taken through my AO/Spencer Series 120 PhaseStar w/ POL and LED ilumination, using a MU300 camera:
Posted 14 September 2014 - 01:03 AM
I've been observing springtails in some "environs" I keep going in my work space. Springtails is a most appropriate name. Love to watch them scurry about on the water surface tension. I have watched as they seem to sense things in the water and they seem to make grabbing motions. I do not know if this is feeding or what. I haven't tried to get any photos yet. Also seeing a number of the brown mites. I'm maintaining my moss & lichen soups in hopes to see some tardigardes, but that hasn't happened yet. I'm doing most of this with a Celestron stereo zoom dissecting microscope.
Time to step away from the microscopes & head back out to the telescopes, needed to warm up some and let the moon get a little higher for better viewing. I've got a new Light Bridge 16" that's getting some lunar time. I tried the LB16 on the moon last month under less than optimal conditions and was stunned!
It's an amazing universe out there no matter which direction you look.