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Where's the photomicrography thread?

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#26 snorkler

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Posted 11 February 2014 - 02:00 AM

It looks great to me, mismatched objectives or not, Michael.

Heres a video I took today. Ciliate twist.

#27 Grava T

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Posted 16 February 2014 - 10:39 PM

Pretty neat!

#28 snorkler

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Posted 03 March 2014 - 09:15 PM

Mosquito larva @40x through the compound scope. Three images stitched because of subject's size.

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#29 snorkler

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Posted 08 April 2014 - 03:27 PM

Found this guy crawling on my newspaper this morning. He's smaller than a period on the newsprint or computer screen.
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#30 snorkler

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Posted 12 April 2014 - 10:14 PM

I just bit on an Amscope T490A-PCT phase contrast trinocular compound scope. It's about 10X the price of my first microscope, and I expect to only have double the pleasure for my money, but I may be surprised.

#31 astronomylife

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Posted 13 April 2014 - 12:34 PM

Nice!, I'm sure you'll enjoy it. I love everything about mine except for the halogen lamp.

#32 snorkler

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Posted 18 April 2014 - 02:25 AM

The T490A-PCT arrived, and it's unusable. The 10x objective goes into darkfield, and won't focus on my blood smear slide. The other three objectives give worse views in phase than in brightfield. I reversed the phase ring turret order, and the 10 & 20x objectives got into phase, but the 40 & 100x gave even worse views. I asked Amscope to ship a better PCT or refund my money.

Testing was done on a commercially prepared blood smear slide. Views of other slides looked fine, but they didn't have subject material that pops in phase.

Follow-up: I called Amscope, and talked to a specialist who said they can ship me a pre-tested PCT. I just emailed my purchase information to Amscope.

#33 snorkler

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Posted 26 April 2014 - 06:03 PM

I returned the defective PCT Thursday. Amscope indicated it may ask me to pay the shipping cost for the replacement PCT. Right after I just paid $20 to ship their defective kit back to them? I don't think so.

Here are some Springtails. I put some damp soil in a funnel with a small glass bottle filled with rubbing alcohol underneath. Then I drove the microscopic Springtails out of the soil with a light bulb placed above the funnel. I'll get better photos when I get an eyedropper or pipette and solidly mount them. This is 40X through the Olympus stereo scope.

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#34 astronomylife

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Posted 28 April 2014 - 12:03 AM

Did you use the T490 to take this picture? How does it compare to the B100?

#35 snorkler

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Posted 28 April 2014 - 12:37 AM

Views through the T490 and B100 are very similar with achro objectives in both. I thought the plan achro phase objective views were sharper at the extreme edges.

I took the Springtail photos through my Olympus SZ4540 stereo microscope. I haven't critically examined photos taken through the T490, as I'm still experimenting.

I considered buying the $90-$100 Amscope DSLR microscope adapter to get parfocal performance in the T490, but the adapter gets poor reviews because of severe chromatic aberration. Above all, I want the best image quality, so I'm using a $15 no-optics Celestron 1.25" telescope adapter shimmed with plastic from a plastic cup. I slide it over the T490's trinocular tube sans eyepiece. I have to focus either for eyepiece or camera. Results should be better than with the B100, because the camera focal plane should be squared more easily with the T490 setup.

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#36 snorkler

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Posted 28 April 2014 - 02:15 PM

Another photo of my T490 photo setup.

More on my PCT. I put 10X achro and Plan achro phase objectives side-by-side in the objective turret, and aligned all four condenser turret annular rings with the phase objective. I compared brightfield and phase views at each condenser turret position. Then I repeated the testing with 40X achro and Plan achro phase objectives

Conclusion: The condenser turret was faulty. Positions 1 and 2 could not give phase contrast views. Phase positions 3 and 4 gave worse views than brightfield, regardless of the phase objective used.

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#37 snorkler

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Posted 06 May 2014 - 05:54 PM

I heard from Amscope today. They received my returned PCT 6 working days ago, and have checked it out. They're testing the replacement PCT before shipping it to me. It should ship today or tomorrow.

#38 snorkler

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Posted 08 May 2014 - 12:01 AM

Friday marks the 30th day Amscope has had $967 of my money, and I haven't had a functional phase contrast kit (PCT).

They didn't ship my replacement PCT today. They've had 6-1/2 working days to check the defective kit they received Apr. 28, and test and send me a working replacement. Tomorrow will be an ugly day for some Amscope employees.

*They shipped the replacement PCT yesterday, but the shipping dept. didn't follow instructions to notify me. ETA is tomorrow.

#39 astronomylife

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Posted 08 May 2014 - 05:26 PM

Looking forward to your opinion of it.

#40 snorkler

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Posted 09 May 2014 - 07:52 PM

Received the PCT this afternoon. Results just as poor as the first PCT, so I guess the problem is in the scope. I'll change the objective positions in the turret, but expect I'll be returning the PCT at a minimum, and possibly the entire combination, come Monday.

Are you in the east or north bay? Maybe I could use your microscope to compare with, and test the PCT with your scope?

#41 snorkler

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Posted 11 May 2014 - 12:27 AM

I used my stepdaughter as a blind tester. She picked the brightfield views as better every time. PCT will be returned. Perhaps the T490, too.

Here are some microscopic insects @200X. Springtails (1 and 2), brown mite, dot bug underside.
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#42 snorkler

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Posted 12 May 2014 - 07:58 PM

Here are some side-by-side brightfield and phase contrast views of epithelial (cheek scrapings). I chose them for their transparency and low contrast, where phase contrast should make them pop. The 100X photos show the PC view is darkfield, and without useful PC effect.
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The 200X photos show the PC view is semi-darkfield, and also without useful PC effect.
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The 400X photos are where the benefits of PC should be most apparent, but I can't see any improvement in details in the right side (PC) photos.
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There are some focusing issues in the photos, but no fine focusing adjustments made the PC views better than the BF views. I did some blind testing with my stepdaughter (an Industrial Biotechnology major) and she unfailingly called the PC views worse than the corresponding BF views.

#43 snorkler

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Posted 29 June 2014 - 10:20 AM

Well, it's time for an update. I shipped the second PCT back to Amscope via USPS, insured for $700, and it never arrived. Worse, I lost my receipt, so I had no proof of insurance.

My Amscope rep went well beyond excellent customer service, and got her manager to give me a courtesy refund for the lost PCT (I kept the T490A). I can't recommend Amscope's PCT, but the company has great customer service!

#44 astronomylife

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Posted 01 July 2014 - 04:08 AM

That's good to hear. After hearing about your trouble with the pct I think I'll stay away from it.

#45 snorkler

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Posted 08 July 2014 - 03:01 PM

Here's a first for me - Escherichia coli bacteria. I don't think I've ever prepared bacterial slides before. I heated the slide to fix the bacteria, used a drop of surplus inkjet printer ink to stain the cells for 15 sec, and blotted the slide dry. The E. coli are the clump of rods below and left of center.

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#46 snorkler

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Posted 08 July 2014 - 03:05 PM

Here's another smattering of E. coli bacteria. 400X for both photos, imaged with a cellphone.

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#47 NGC704

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Posted 09 September 2014 - 12:14 PM

Here's a comparison between normal bright field, and bright phase contrast (as opposed to dark phase contrast - yes, I have both), using a living diatom at 200X for a subject. First, bright field:

 

2013-08-03_0029_zpsac5600c1.jpg

 

And phase contrast:

 

2013-08-03_0031_zps05443cad.jpg

 

Polarized light is a ton of fun, here's some Galveston beach sand with a couple forams (microscopic sea shells) in POL at 100X:

 

12_13_13100X02_zps67bee0dd.jpg

 

Observing sand is a lot like observing the sky; just as we occasionally run across unique stars among the myriad others, there are unique sand grains, like this cross-striated one seen at 400X:

 

12_13_13400X04_zps1994b137-1-1.jpg

 

How about a little sugar with that? Here's some table sugar that was dissolved in tap water, then a drop allowed to dry on a slide, then observed under POL at 100X:

 

12_15_13TableSugar100XPOL_zps27b685fa.jp

 

Similar treatment applied to a Vitamin C tablet produced this crystal structure seen under POL at 100X:

 

12_15_13VitaminC100XPOL_zps7d194e79.jpg

 

Photos above taken through my AO/Spencer Series 120 PhaseStar w/ POL and LED ilumination, using a MU300 camera:

 

2_14_14AO120PCPOL1600_zps64e91e0f.jpg



#48 kozmik frakture

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Posted 14 September 2014 - 01:03 AM

I've been observing springtails in some "environs" I keep going in my work space.  Springtails is a most appropriate name.  Love to watch them scurry about on the water surface tension.  I have watched as they seem to sense things in the water and they seem to make grabbing motions.  I do not know if this is feeding or what.  I haven't tried to get any photos yet.  Also seeing a number of the brown mites.  I'm maintaining my moss & lichen soups in hopes to see some tardigardes, but that hasn't happened yet.  I'm doing most of this with a Celestron stereo zoom dissecting microscope.

 

Time to step away from the microscopes & head back out to the telescopes, needed to warm up some and let the moon get a little higher for better viewing.  I've got a new Light Bridge 16" that's getting some lunar time.  I tried the LB16 on the moon last month under less than optimal conditions and was stunned!

 

It's an amazing universe out there no matter which direction you look.








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