I thought I just mastered taking my first flats about 3 weeks ago, and in fact my flats did remove the vignetting from my image frames taken with the ATIK 383L (Kodak 8300 chip with 1.25" filters). I stretched the newly processed light frames after applying flats and I couldn't stretch to reveal any vignetting on all four corners like I did without taking flats so I deemed that to be a success. But a few nights ago, the skies were "clear" but the relative humidity was 97%. After a 4 hour session of imaging the Cave Nebula I brought my scope in to the basement and took all the flats and applied them. The next morning I started to process them in Nebulosity3 as I always do and my processed subframes still had vignetting despite applying flats and they almost looked like the original lights before applying the flats. The only thing different between the session 3 weeks ago and the one a few days ago was heavy dew. I was using my 80mm apo with dew shield so I didn't bother to check the lens for any moisture. I'll have to make sure to check next time we have a heavy dew.
So my question is, "Can a very high humidity (it was 97% according to my outdoor weather station sensor) increase the effect of vignetting, as opposed to a dry night?" Any answers greatly appreciated.