Do have appropriate clothing on or nearby.
Don't assume that you'll be as comfortable at 2am in what you were comfortable wearing at 8pm.
Do gain familiarity with your equipment, and have it pretty well collimated before venturing out.
Don't assume that it came in optimal condition just because it's new.
Do plan your observing session, or at least become familiar with what you are likely to see, and in what part of the sky, and at what time, prior to your observing session.
Do familiarize yourself with your charts and atlas before expecting to use them in the field with a red light.
Do have all your accessories collected and organized, and
Do remember to bring them.
Do remember to bring fresh batteries for anything that requires batteries.
Depending on what type scope you have,
Do remember to bring a stool or chair to observe with. Even if you don't use it for observing, (which is highly recommended if possible), you may find that sitting occasionally really helps, anyway.
Do keep your expectations low and reasonable.
Don't expect Hubble images, and
Don't expect to see unreasonable numbers of objects in one session.
Do expect your observing skills to increase with time.
Don't expect to start right out seeing to the limits of your equipment.
Do spend a good amount of time really learning the limits of your equipment before upgrading or changing.
Do take advantage of the generally better seeing conditions from midnight to dawn.
Do take advantage of dark sky sites as often as possible
Do take advantage of any local astro clubs if possible
Do remember to relax and have fun.