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Methylene Blue microscopy stain

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#1 proud uncle

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Posted 25 July 2015 - 11:56 PM

Is Methylene Blue packaged for use in fish aquariums the same stuff as that packaged for use as a microscope stain?  In other words, can I use MB for fish aquariums as a MB microscopy stain?

 

Thanks,

Kenneth



#2 chrysalis

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Posted 26 July 2015 - 06:00 AM

yes (except maybe the concentration required)



#3 Otto Piechowski

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Posted 10 August 2015 - 10:54 AM

On this topic of Methylene Blue, I have a laboratory grade flask (small) of the dry powder.  For microscopy purposes, should I mix this power with alcohol or with distilled water?  At what concentrations/parts per substance, should they be mixed?

 

Thank you.

 

Otto



#4 Otto Piechowski

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Posted 11 August 2015 - 06:18 PM

All the years of sharing comments with you in the astronomy forums, EJN, only to happily discover you are here as well!

 

The distilled water, ethanol, and methylene blue; what those are and the proportions to mix I can handle.  But what are and where does one find potassium hydroxide (?KOH) or sodium hydroxide (NaOH)?

 

The formula you found for me may explain why all these years my methyl blue stains have been good, but nothing fantastic.

 

Otto



#5 Michael Covington

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Posted 11 August 2015 - 07:54 PM

NaOH is lye, sold as a drain cleaner in hardware and grocery stores.  Be careful with it.



#6 Michael Covington

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Posted 11 August 2015 - 07:55 PM

By the way, quite a few dyes, inks, etc., can be used as microscopy stains if you don't need complete equivalence to traditional stains.  Gentian violet and tincture of iodine (from the drugstore) are useful (diluted).



#7 Otto Piechowski

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Posted 12 August 2015 - 06:33 PM

I appreciate your help.

 

In Loeffler's mixture/formula which EJN provided us, the statement is made "1 mL dilute (1%) KOH or NaOH".  How would/what would be the parts used in making this "(1%)".  Would it be 1 gram of NaOH to 100 grams of distilled water?  And if that is correct, is a milliliter of water equivalent to a milligram of water?

 

Thank you.

 

Otto



#8 Otto Piechowski

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Posted 13 August 2015 - 11:47 AM

thank you EJN.  




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