PRNU stands for "Pixel Response Non-Uniform". It represents a variation in pixel responsivity across the array and is normally expressed as a percentage of mean responsivity. (Responsivity is similar to QE; however, it has different units. It converts irradiance into Amps [or micro-Amps] as the output of a detector [i.e. a pixel] The expression for signal is: S(m,n) = R(m,n) A I(m,n) I(m,n) where R(m,n) is the responsivity of a pixel at location (m, n) in units of Amps/Watt, A = the pixel area in m2, and I(m,n) is the average irradiance over pixel(men) in Watts/m2.) A typical value for PRNU might be 1%. You can't see PRNU directly. Instead you see the effect of PRNU on a signal and that effect is call Fixed Pattern Noise (FPN.)
John:
Quick correction. For the signal equation you put irradiance in there twice. Should be S(m,n) = R(m,n) A I(m,n), and using your MKS units the signal S(m,n) comes out as [A] or [C/s]. One can convert that to [e-/s], which is commonly used in this line of work as related to camera sensors.
All:
John made the point above that demonstrates one of the distinctions between FPN and Shot Noise as the former increases linearly with signal, the latter as the square root with signal. This can be seen when constructing a Photon Transfer Curve (PTC) of a camera sensor. Make a log-log plot of FPN and Shot Noise vs. Signal, and through the linear response region of the sensor the former will have a slope of +1, the latter +1/2 when the data is properly calibrated. John gave references to Janesick and Crisp for details on how to do this. That +1 shows the direct relation of FPN with signal illumination as has been discussed, the +1/2 denotes the poissonian distribution of shot noise.
I bring up the PTC because learning to make these with those references as guide was the most constructive way for me to learn about all these terms and better understand their sources, how they interrelate and, most importantly, how and why I really need to properly calibrate with dark subtraction and flat-fielding as well as the benefit of dithering. For me the exercise helped to set my mind straight and use better practices.
Some of these debates have been going on for quite some time, but I still recommend that we all pay attention to them and listen and learn and do our best to anchor the concepts properly. But at the same time, putting the concept debates aside, note there is near universal agreement on the necessity of proper flat-fielding calibration as well as the strong recommendation of dithering. I wouldn't re-emphasize this last point if not for the fact that even today I came across yet another post (no need to link to it since this was a beginner who I'm sure will learn in time) where the imager stacked uncalibrated frames and tried to post-process his way to a better result. I for one will continue to gently advise not to do that! And I'm happy to explain why using to the best of my ability proper terminology.
Lastly, good job to the OP for working his way through this. Your last calibrated image was quite good with maybe a slight bit of roll-off over- (or was it under-?) correction. I suffer from that often, although yours is minimal. It's also possible you just have some sky gradient to deal with. (Now, sky glow I know for sure is signal, albeit unwanted signal!) I look forward to seeing your finished images.
Best Regards,
Ben