The problem with the 5100 is entirely different. The black point is set at zero, which chops half your bias off. Plays havoc with calibration. The hack moves it to 600ADU, and the D5300 is already there.
ISO 100 seems to have quirks. I use 200, and do so consistently, it's general purpose.
There's no need for dark flats for the D5300/5500/5600. Bias works fine. This is also true for many cameras, the ZWO1600 (which does need dark flats) seems to have caused an overreaction spilling onto cameras which do not need dark flats.
Should we just use one though? As in, feed my master ISOxxx bias into DSS, or use DF's, but not both? I had been using every calibration frame DSS allowed, but recently tried out just DF's. It seemed better, but I could be fooling myself of course. Looking at the formula DSS says it uses, it would seem in that when you use everything the bias just ends up washing out anyway, but I'm not real sure.