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Fixin' to Start: Surveying the Fields in Microscopy

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#26 vertex2100

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Posted 05 March 2022 - 02:14 AM

Zeiss APO 100X, N.A .1.32 pH 3 objective.  Again, shaky, resized cell phone pictures, so looks vastly better in person.  

Screenshot_20220305-094043_Gallery.jpg

Screenshot_20220305-094242_Gallery.jpg Screenshot_20220305-094135_Gallery.jpg Screenshot_20220305-100750_Gallery.jpg


Edited by vertex2100, 05 March 2022 - 02:23 AM.


#27 vertex2100

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Posted 05 March 2022 - 02:33 AM

And, like Brian suggested,  good to eventually have a phase contrast outfit and at least a simple polarizing setup to see basic  polarizing effects. 

Screenshot_20220305-102856_Gallery.jpg Screenshot_20220305-102905_Gallery.jpg Screenshot_20220305-102913_Gallery.jpg



#28 mikemarotta

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Posted 05 March 2022 - 07:06 AM

Yes, Mike, if you want the most certainty of proper functionality,  then you should buy one of the new ones recommended by Oliver Kim.  For me, I like the craftsmanship and solidity of the older scopes. ... My daughter's fish tank sediment.  

 

Yes, I certainly appreciate the old craftsmanship. For me, that is printing. (See "For the Glory of Old Lincoln High" on my blog, NecessaryFacts.) Having learned to set type by hand in a shop class while I learned to count headlines in my first journalism class made the desktop printing revolution an easy wave to ride. I have two sliderules on my bookshelf (and others in a box). So, I understand your passion. 

 

And, please pardon the direct reply, but the pictures you post of microscopic things are pointless. What are they? If you do not understand what you are looking at, why bother? It is very much like the astrophotographers here who post rosier Rose Nebulae that tell us nothing about about the astrophysical processes. I am just not sold on it. To me, photography is recording. In science that is the recording of fact. As I understand things, you need to identify the facts or you just have abstract art.

 

Irreproducible Results JIR cost saving.jpg

 

The Journal of Irreproducible Results was a humor magazine for scientists. Launched as a mimeographed samizdat in 1955, it remained popular for over 45 years. “A Drastic Cost Saving Approach to Using Your Neighbor’s Electron Microscope” by Aalbert Heine offered a photomicrograph that was captioned “Fig. 1: A eutectic mixture of quartz and plagioclase … Fig. 2: A cross section through the skin of a peripatus … Fig. 3: The surface of a root hair of a quadruploid species of crabgrass. … Fig. 4: Fragment of a hickory ax handle. …”

 

I would be happy to buy a classic microscope if I knew what I was getting. That is why I came here to this Forum and started this and another topic and it is why I participate on Microbe Hunter. (BTW, I worked for Zeiss in the 90s, as a trainer and technical writer in their industrial metrology division. I also translated documents from German to English.) 


Edited by mikemarotta, 05 March 2022 - 07:41 AM.


#29 vertex2100

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Posted 05 March 2022 - 12:35 PM

The photo of the fish tank sediment shows some diatoms. Diatoms are often used to test the resolution of an objective. The polarizing microscope photos show the crystals of ethylenediaminetetraacetic acid  EDTA that are inside the bottle of it first pictured. It has many uses including chelating heavy metals in people's blood. We use it in dentistry to help clean the insides of root canals.The second polarizing photo shows the effects of a 1st order red plate on the same sample.  I'm sure you will be happy and see lots of interesting things no matter which microscope you buy. 



#30 vertex2100

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Posted 05 March 2022 - 12:37 PM

He should have also put the choices of tooth brush bristles and bundle of spaghetti.  




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