All good replies, but I want to emphasize again that seeing really has no effect on tracking and thus given the title of this thread it's not really a factor. However, seeing definitely will have an effect on your image quality, the smallness of your stars, their number, and to some extent their shape. Plus, seeing will definitely impact GUIDING and for that reason it's always harder to guide when the seeing is poor.
Thus, we have two different questions. Does seeing affect your tracking? The answer is no. Does seeing affect the quality of your results? The answer is yes.
Note that the effects of seeing are kind of independent of your target's altitude. Thus, on a night will really poor seeing your results when imaging near to the zenith could be just as bad as when imaging near to the horizon on a night with very good seeing. So, it's the quality of the seeing and not necessarily the target's altitude that is the major factor in overall image quality (if your ignore atmospheric refraction and dispersion). Of course, on any give night (or hour) your seeing will always be worse when imaging near to the horizon but again that has no real effect on your tracking (just as it would have no effect on the results when tracking near to the zenith).
The only factors that affect tracking are the quality of your mount, your polar alignment, and atmospheric refraction and all three of these factors will depend on where in the sky you are imaging (both in altitude and direction).
All that said, seeing does play a MAJOR role in the quality of your results so for that reason alone it's usually best to avoid imaging when near to the horizon. Similarly, depending on the quality of you mount and the length of your exposures it may actually be a good idea to disable guiding when the seeing is really poor because your tracking might be better than your guiding.
However, unless you have a premium mount with absolute encoders and a well-calibrated sky pointing model I wouldn't advise doing any exposures longer than about 30 seconds (but this depends upon your image scale -- your focal length and pixel size). Is it possible to go longer than 30 seconds when NOT guiding? I'd say yes but that would require a fairly modest image scale with a "decent" mount, a good polar alignment, and when imaging fairly high in the sky.
In any case, the results reported by the OP are probably the result of both tracking and seeing where seeing would certainly have the potential for the cause of most of their quality issues.
Edited by james7ca, 01 April 2023 - 10:34 PM.