I am still learning how to assemble sequences in NINA and making some progress. My process, prior to running the sequence, is to polar align, slew somewhere near the meridian and equatorial arc and run auto focus, then start guiding with PHD2 and run Calibration. Once complete I let it guide for 10-15 minutes to make sure everything appears to be working. Then I stop guiding and Park the telescope. Then I run the sequence which because I have limited night sky, usually includes a wait for time instruction before starting.
This has worked well with a single target. Once the Sequence fires, it goes to the target, runs the instructions, ends up with Parking again. Guiding is good, sometimes excellent.
Several nights ago, I tried doing two targets in one sequence. The first night, it was a direct slew from one target to the other once imaging was done on the first. I did stop guiding prior to the slew, captured, did an autofocus and then commanded the guiding back on. The guiding on the second target did not work. I had to interrupt the sequence, manually invoke a recalibration, and then it worked. I thought maybe it was an anomaly.
Last night I set up two targets again. This time, I parked the scope between targets for about two hours and used a wait until time command to bring it out of park and start the process on the second target. Again, the guiding failed. I paused the sequence, called the calibration routine and then restarted the sequence and all was fine.
I was under the impression that once polar aligned correctly and with PHD2 calibrated, it should run successfully. Particularly if the targets are both on the same side of the meridian, and these both were on both nights. In fact, I thought I read you could reuse an existing calibration. I have limited experience with a Meridian Flip as most of my visible sky is west of the meridian. However, I have done a few and guiding has worked well from a single calibration on both sides of the meridian.
I think I can solve this within the Sequence by "forcing" a recalibration prior to the second target. I will try that tonight. But I am interested in understanding the limitations of the initial calibration to subsequent targets in the same sequence and what behavior I need to accommodate within a sequence.
This is an IOptron GEM 28 and is not overloaded. Most nights I get what I think are exceptional guiding. I am Bortle 5. Once calibrated, I normally see guiding numbers of ASC (.08 to .14) and DEC (.08 to .12) and total of .14 - .18. Stars are round and on a good seeing night, autofocus might not fire for long periods. In both instances, the initial target was fairly high in the sky and the second target was near the meridian and DEC about -15.